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Figure 5. Sildenafil down-regulates IL-4R expression on CD11b+ cells. CD4+, CD8+, CD11b+, or CD11c+ cells were magnetically purified from spleens of C26GM-bearing mice challenged 9 d earlier. CD4+ or CD8+ T cells were stimulated with ConA for 3 d, with or without 50 µg/ml sildenafil. (A) Purified cells were cultured separately for 2 d with or without sildenafil, harvested, and used to determine cGMP levels, as described in Materials and methods. (B) T cell proliferation was determined by [3H]thymidine incorporation. (C) CD11c+ cells were analyzed for B7.2 and MHC class II expression. (D) CD11b+ cells were analyzed for IL-4R expression by flow cytometry. The t test p-value is reported. Error bar values are shown. MFI, multiplicity of infection.
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